DNA
Part:BBa_K3765009
Designed by: Dipayan Akhuli Group: iGEM21_IISc-Bangalore (2021-10-19)
22 Amino Acid Linker
Flexible 22 amino acid linker (LEGSGQGPGSGQGSGSPGSGQG) that can be used to space out two domains in a fusion protein so that the domains do not interfere with each other and fold independently.
Usage and Biology
The first 15 amino acids were taken from Bisson-Filho et al. (Science, 2017), while the other 7 amino acids consisted of similar repeated motifs as the first 15 amino acids. This long linker was used in our project to space out the OpdA enzyme from the SpyTag002 module in the OpdA-SpyTag002 family of fusion proteins, in order to preserve the folding and hence the activity of the enzyme as well as the isopeptide bond formation ability of the SpyTag002 with SpyCatcher002.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//proteindomain/internal
//proteindomain/internal/special
//proteindomain/linker
//proteindomain/internal/special
//proteindomain/linker
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